Department of Microbiology: Online Lab Manual

Parasitology Manual

Over 70 different species of parasites, belonging to two major groups (Protozoa and Helminths), can be found in various parts of the human body.

Parasitosis may result from exposure via one or more of the following sources: 1) contaminated soil or water; 2) food containing the immature infective stage of the parasite; 3) a blood sucking insect; 4) a domestic or wild animal harboring the parasite; 5) another person, their clothing, bedding, or the immediate environment that they have contaminated; or 6) oneself.

Competent laboratory work is dependent on several factors; 1) satisfactory specimens; 2) safe and adequate facilities, including a good quality microscope; 3) personnel trained in examining specimens and accurately identifying organisms; and 4) personal trained in safety and protection from stool, body fluid and blood-borne pathogens (Universal Precautions).

The most common types of body material submitted for parasitology examination are stools and blood, however other materials, such as anal swabs, urines, aspirates, abscesses or respiratory specimens, surgical specimens and biopsies may all be submitted in certain cases.

Disclaimer: These documents have been developed specifically for our institutions and may not be appropriate for implementation in other settings. This information is made available for professional education purposes only and each piece of information should be carefully evaluated before being adapted to meet the needs of other facilities or settings.

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Title
MI\PARA\01
Introduction
MI\PARA\02
Laboratory Safety Guidelines
MI\PARA\03
Turnaround Times (TAT)
MI\PARA\04
Collection And Preservation of Stool Specimens
  Laboratory Procedures for Stool Examination
MI\PARA\05\01

» Introduction

MI\PARA\05\02

» Procedure: Examination of Stool

MI\PARA\05\03

» Direct Wet Preparation Of Fresh Stools

MI\PARA\05\04

» Formalin-ether Concentration Method

MI\PARA\05\05

» Permanent Stained Smear Methods

MI\PARA\05\06

» Hematoxylin Stain

MI\PARA\05\07

» Smears For Coccidia

MI\PARA\05\08

» Smears For Microsporidia (Modified Trichrome or Chromotrope Stain)

MI\PARA\05\09

» Sequential Haematoxylin/Acid Fast Stain For Routine O & P Including Cryptosporidium & Cyclospora

MI\PARA\05\10

» Hatching Schistosoma Eggs

MI\PARA\05\11

» Kato Thick Smear For Egg Count

MI\PARA\05\12

» Agar Plate Test for Strongyloides

MI\PARA\05\13

» Charcoal Culture for Strongyloides

MI\PARA\05\14

» Baermann Technique for Strongyloides

  Collection And Laboratory Procedures For Specimens Other Than Stool Or Blood
MI\PARA\06\01

» Anal Swabs for Pinworm Eggs

MI\PARA\06\02

» Duodenal Aspirates

MI\PARA\06\03

» Urines

MI\PARA\06\04

» Vaginal and Urethral Swabs

MI\PARA\06\05

» Sigmoidoscopy

MI\PARA\06\06

» Lung and Liver Aspirates

MI\PARA\06\07

» Hydatid Cysts

MI\PARA\06\08

» Skin Biopsy

MI\PARA\06\09

» Sputum

MI\PARA\06\10

» Lymph Nodes

MI\PARA\06\11

» Muscle Biopsy

MI\PARA\06\12

» Rectal or Bladder Biopsy Smears for Coccidia

MI\PARA\07
Reporting Results Of Enteric Parasitology Specimens
MI\PARA\08
Cultivation Of Intestinal And Urogenital Protozoa
MI\PARA\09
Collection Of Blood Specimens
MI\PARA\10
Protocol For Malaria Smears
  Laboratory Procedures For Blood And Tissue Parasites
MI\PARA\11\01

» Malaria - Plasmodium spp. and Babesia spp.

MI\PARA\11\02

» Field’s Stain

MI\PARA\11\03

» Leishmania spp.

MI\PARA\11\04

» Microfilaria in Blood

MI\PARA\11\05

» Microfilaria Isolation on Nucleopore Filters

MI\PARA\11\06

» Hematoxylin Stain for Microfilaria

MI\PARA\11\07

» Toxoplasma gondii

MI\PARA\12
Laboratory Procedures for Leprosy
MI\PARA\13
Action Plan
MI\PARA\14
Quality Assurance Procedures For The Parasitology Laboratory
MI\PARA\15
Performance Standards: Parasitology Laboratory
MI\PARA\16
Retention Times
MI\PARA\17
References
MI\PARA\18
Appendices
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MI\PARA Download entire Parasitology Manual in PDF format
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