The following procedure and protocol manuals are provided for
our microbiology laboratory and are available as an educational
reference for other laboratories. All manuals are in PDF format,
and will require installation of Adobe
Acrobat Reader to view, if not already installed.
Disclaimer:
These documents have been developed specifically
for our institutions and may not be appropriate for implementation
in other settings. This information is made available for professional
education purposes only and each piece of information should be
carefully evaluated before being adapted to meet the needs of other
facilities or settings.
» General Manuals
Manual |
Summary |
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Details about all the available microbiology
tests from our lab are contained in this searchable database.
For more information regarding pricing and services, please
visit the Toronto
Medical Laboratories website. |
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The main objective of this training program
is to produce a technologist who has the ability to apply
theoretical knowledge to the practical aspects of the Microbiology
laboratory, efficiently and with a caring attitude. |
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A collection of important process control
documents that are frequently referred to by laboratory staff. |
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The Laboratory Safety manual is intended
to address universal safety measures for achieving a safe
and healthy working environment. It describes good laboratory
practices that must be understood and observed by all individuals
involved in the laboratory. It describes control measures
essential for protecting all laboratory occupants from potential
biological, chemical and physical hazards. |
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The purpose of the quality manual describes
the laboratory's quality management system. Quality Management
System encompasses all management activities and processes
relating to:
organization, personnel, equipment, purchasing and inventory,
process control, document and records, information management,
occurrence management, process improvement, service and satisfaction
and facilities and safety. |
» Reference
Manuals
Manual |
Summary |
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Different organisms will require different
methods of susceptibility testing. This manual outlines the
methodologies and procedures of vitek, disk diffusion, beta-lactamase,
oxacillin, and other methods. |
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Work-up for aerobic bacteria, anaeroblic
bacteria, and yeast. |
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Numerous diagnostic tests and procedures
used in the microbiology lab. |
» Bench Manuals
Manual |
Summary |
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The recent events in the USA have created
a heightened awareness and concern regarding the potential
of a bioterrorist attack. Because such an attack could be
overt (announced/broadcast) or covert, the microbiology laboratory
may play an important role in the initial identification and
control of spread of potentially infectious agents. |
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Although this section is mainly directed
towards the processing of blood cultures, occasionally other
specimen types (eg. Sterile fluids, Bone marrow, abscess material)
are received in blood culture bottles and thus their processing
and work-up will be described. The current blood culture system
used in the Microbiology Laboratory is the non-radiometric
BacT/Alert System. |
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Procedures on reporting, specimen collection,
specimen rejection, specimen transport, and reporting on faeces/rectal
swaps, duodenal or small bowel aspirates/biopsies/swabs, and
rectal/large bowel biopsies. |
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Methods used to isolate or detect organisms
which are associated with infection or disease of the genital
tract. |
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Procedures on for testing VRE, MRSA, EBSL's,
and antibiotic resistant organisms. |
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Methods used to isolate, identify, and
report mycology samples. |
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Quality Control methods including Vitek
QC, Kirby Bauer QC, E-test QC, and Serology QC. |
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Procedures including bronchoscopy specimens,
epiglottal swabs, lung biopsies, mouth swabs, nasal swabs,
and throat swabs. |
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Serological testing procedures including
Epstein-Barr, Hepatitis B, Cytomegalovirus, Herpes, and HIV. |
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Sterile fluid testing procedures including
cerebrospinal , pleural, peritoneal, amniotic, and transfusion
products. |
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Procedures used to check for sterility
in samples, including those from the bone bank, environmental
specimens, pharmacy, distilled water, and air. |
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Urine specimens for culture are collected
when the following syndromes are suspected: cystitis, pyelonephritis,
asymptomatic bacteriuria, and less commonly acute prostatitis,
pyelonephric abscess, and urosepsis. |
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Diagnostic Virology is performed for a
variety of reasons, ranging from the etiologic diagnosis of
an acutely ill patients’ disease to retrospective serologic
surveys for evidence of past infection. |
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Instructions on how to use the Vitek system. |
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Procedures and methods for skin, wound,
tissue, ear, eye, and aspirate specimens. |
This website has been made possible through an unrestricted educational grant from
Pfizer Canada Inc. |
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