RT-PCR
benefits and drawbacks
üDetection
of small quantities of target RNA in clinical samples
üReal-time detection
üUse of probe adds specificity
ü
ü
û
ûChance of
carry-over contamination
ûNeeds a thermocycler
ûMay still fail to pick-up low viremia in asymptomatic
patients especially if use minipools
û
û
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