Department of Microbiology: Online Lab Manual

Mycology Manual

Methods used to isolate, identify, and report mycology samples.

Disclaimer: These documents have been developed specifically for our institutions and may not be appropriate for implementation in other settings. This information is made available for professional education purposes only and each piece of information should be carefully evaluated before being adapted to meet the needs of other facilities or settings.

Policy #
Title
MI\MYC\01
Lab Safety
MI\MYC\02
Daily Routine Of The Mycology Lab
QPCMI02001
Pre-analytical Procedure - Specimen Collection
QPCMI06003 Specimen Processing Procedure
MI\MYC\04
Isolation and Identification
MI\MYC\05
Reporting
  Staining Methods
MI\MYC\06\01

» Acid-Fast Stain For Nocardia (Modified Kinyoun)

MI\MYC\06\02

» Calcofluor White Stain

MI\MYC\06\03

» Fungi-Fluor™ Stain

MI\MYC\06\04

» Gomori Methenamine Silver (GMS) AND Periodic Acid Schiff (PAS) Fungal Stains

MI\MYC\06\05

» India Ink

MI\MYC\06\06

» Lactophenol Aniline Blue (LPAB)

MI\MYC\07
Isolator 10 Blood Culture System For Dimorphic Fungi
MI\MYC\08
References
  Appendices
MI\MYC\10\01

» Appendix I: Conversion (Converting Mycelial Phase of Dimorphic Mould to a Yeast Phase)

MI\MYC\10\02

» Appendix II: Cornmeal Tween-80/Oxgall Agar

MI\MYC\10\03

» Appendix III: Determining Cycloheximide Resistance Of An Isolate

MI\MYC\10\04

» Appendix IV: Fluorescent Microscope (Instructions)

MI\MYC\10\05

» Appendix V: Germ Tube Test

MI\MYC\10\06

» Appendix VI: Slide Culture

MI\MYC\10\07

» Appendix VII: Stock Cultures -Water Culture Technique

MI\MYC\10\08

» Appendix VIII: RAPI 20 CAUX - Yeast Identification System

  Appendix X: Media / Reagents
MI\MYC\09\01

» Blood Egg Albumin Agar (BEAA)

MI\MYC\09\02

» Brain Heart Infusion Agar

MI\MYC\09\03

» Cornmeal Tween 80 Agar (Oxoid)

MI\MYC\09\04

» Esculin Base Medium (EBM) pH 7.1

MI\MYC\09\05

» Inhibitory Mold Agar (IMA)

MI\MYC\09\06

» Lactophenol Aniline Blue Stain (LPAB)

MI\MYC\09\07

» Mycosel Agar

MI\MYC\09\08

» Oxgall Agar

MI\MYC\09\09

» Potato Dextrose Agar (PDA)

MI\MYC\09\10

» Sodium Pyruvate Agar (NPA) for Nocardia

MI\MYC\09\11

» Sabouraud Agar Modified (DIFCO)

MI\MYC\09\12

» Sabouraud Gentamicin Agar Slopes

MI\MYC\09\13

» Urea Agar Slopes

MI\MYC\09\14

» 10% Potassium Hydroxide

-  
MI\MYC Download entire Mycology Manual in PDF format
This website has been made possible through an unrestricted educational grant from Pfizer Canada Inc.
Copyright 1999-2007 Department of Microbiology, Mount Sinai Hospital, Toronto, Canada. All rights reserved.